Frattini, Domenico; Hyun, Kyuhuan; Kwon, Yongchai published the artcile< Direct electrochemistry of lactate dehydrogenase in aqueous solution system containing L(+)-lactic acid, β-nicotinamide adenine dinucleotide, and its reduced form>, HPLC of Formula: 606-68-8, the main research area is lactic acid nicotinamide adenine dinucleotide lactate dehydrogenase electrochem.
Using lactate dehydrogenase (LDH) for lactate detection in biosensors is fascinating but still challenging because in-situ bioelectrochem. sensors are still at the research stage due to the lack of reliable information about the direct electrochem. of systems not involving redox mediators. To provide reliable information, the direct electrochems. of aqueous solution systems containing L(+)-lactic acid (Lac), β-NAD (NAD+) and its reduced form (NADH) is investigated using (i) free-standing LDH and (ii) LDH immobilized on carbon nanotubes (CNT). Results show that there is one peak for NADH oxidation and one peak for the NAD+ reduction while the conversion of lactate is observed in the aqueous solution In addition, the peak for NADH oxidation may disappear or be shifted irreversibly. As a result, the detection of lactate relies on the type of solution The conventional mechanisms explaining these phenomena are too superficial and hard to interpret, thus, in this work, we suggest a new interpretation method explaining the empirical behavior of lactate detection. In this prospect, the role of chem. equilibrium related to the conversion of lactate into pyruvate that is catalyzed by LDH is important and this is sequentially elucidated, while the rate-determining step for lactate detection is suggested.
Journal of Industrial and Engineering Chemistry (Amsterdam, Netherlands) published new progress about Carbon nanotubes. 606-68-8 belongs to class chiral-phosphine-ligands, and the molecular formula is C21H27N7Na2O14P2, HPLC of Formula: 606-68-8.
Referemce:
Phosphine ligand,
Chiral phosphine ligands in asymmetric synthesis. Molecular structure and absolute configuration of (1,5-cyclooctadiene)-(2S,3S)-2,3-bis(diphenylphosphino)butanerhodium(I) perchlorate tetrahydrofuran solvate