Carrasco, Begona; Moreno-del Alamo, Maria; Torres, Ruben; Alonso, Juan Carlos published the artcile< PcrA dissociates RecA filaments and the SsbA and RecO mediators counterbalance such activity>, Electric Literature of 606-68-8, the main research area is PcrA RecA filaments SsbA RecO mediator counterbalance activity; ATPase; RecA; RecO; SsbA; UvrD; strand exchange.
PcrA depletion is lethal in wild-type Bacillus subtilis cells. The PcrA DNA helicase contributes to unwinding RNA from the template strand, backtracking the RNA polymerase, rescuing replication-transcription conflicts, and disassembling RecA from single-stranded DNA (ssDNA) by poorly understood mechanisms. We show that, in the presence of RecA, circa one PcrA/plasmid-size circular ssDNA (cssDNA) mol. hydrolyzes ATP at a rate similar to that on the isolated cssDNA. PcrA K37A, which poorly hydrolyzes ATP, fails to displace RecA from cssDNA. SsbA inhibits and blocks the ATPase activities of PcrA and RecA, resp. RecO partially antagonizes and counteracts the neg. effect of SsbA on PcrA- and RecA-mediated ATP hydrolysis, resp. Conversely, multiple PcrA mols. are required to inhibit RecA·ATP-mediated DNA strand exchange (DSE). RecO and SsbA poorly antagonize the PcrA inhibitory effect on RecA.ATP-mediated DSE. An iterative translocating PcrA monomer strips RecA from cssDNA to prevent unnecessary recombination with the mediators SsbA and RecO balancing such activity; and a PcrA cluster that disrupts DNA transactions, as RecA-mediated DSE.
Frontiers in Molecular Biosciences published new progress about Bacillus subtilis. 606-68-8 belongs to class chiral-phosphine-ligands, and the molecular formula is C21H27N7Na2O14P2, Electric Literature of 606-68-8.
Referemce:
Phosphine ligand,
Chiral phosphine ligands in asymmetric synthesis. Molecular structure and absolute configuration of (1,5-cyclooctadiene)-(2S,3S)-2,3-bis(diphenylphosphino)butanerhodium(I) perchlorate tetrahydrofuran solvate